产品属性:
产品名称 | DABCYL-γ-Abu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS |
规格 | 1mg、5mg |
货号 | EY-01Y11913 |
Cas No.: 142988-22-5
别名: N/A
化学名: N/A
分子式: C90H120N22O16S
分子量: 1798.15
溶解度: Soluble in DMSO
储存条件: Store at -20°C
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述:
The fluorogenic peptide substrate DABCYL-Abu-HPFHLVIHT-EDANS has been developed to continuously measure the proteolytic activity of human renin (Km = 1.5 µM at physiological pH). Cleavage of the FRET substrate occurs specifically at the Leu-Val bond and corresponds to the renin cleavage site of angiotensinogen. By means of this assay as low as 30 ng/mL renin can be detected after an incubation of only 3-5 min. Enzymatic cleavage yields the EDANS-fragment M-2645.
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