上海一研生物科技有限公司

产品属性：

Cas No.: 957-75-5

别名: N/A

化学名: N/A

分子式: C9H11BrN2O6

分子量: 323.1

溶解度: DMF: 10mg/mL,DMSO: 15mg/mL,PBS (pH 7.2): 5mg/mL

储存条件: Store at -20°C
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.

Shipping ConditionEvaluation sample solution : ship with blue ice

All other available size: ship with RT , or blue ice upon request

产品描述：

5-Bromouridine is a brominated analog of the nucleoside uridine .1 It can be incorporated into RNA and subsequently detected by antibodies against bromodeoxyuridine .2 5-Bromouridine decreases the viability of HL-60 and MOLT-4 cells (LC50s = 10 and 20 μM, respectively). It induces apoptosis and halts the cell cycle at the S phase in HL-60 cells. It is photoreactive, and UV irradiation has been used to cross-link RNA containing 5-bromouridine to proteins in the study of of RNA-protein interactions.1,3 5-Bromouridine can also be incorporated into RNA using 5-bromouridine 5'-triphosphate .1.Tanner, N.K., Hanna, M.M., and Abelson, J.Binding interactions between yeast tRNA ligase and a precursor transfer ribonucleic acid containing two photoreactive uridine analoguesBiochemistry27(24)8852-8861(1988)

2.Li, X., Patel, R., Melamed, M.R., et al.The cell cycle effects and induction of apoptosis by 5-bromouridine in cultures of human leukaemic MOLT-4 and HL-60 cell lines and mitogen-stimulated normal lymphocytesCell Prolif.27(6)307-319(1994)

3.Gott, J.M., Willis, M.C., Koch, T.H., et al.A specific, UV-induced RNA-protein cross-link using 5-bromouridine-substituted RNABiochemistry30(25)6290-6295(1991)
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