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产品属性：
产品名称 | 5-Cyano-2,3-di-(p-tolyl)tetrazolium (chloride) |
规格 | 10mg、25mg、50mg、100mg |
货号 | EY-01Y13595 |
Cas No.: 90217-02-0
别名: N/A
化学名: N/A
分子式: C16H14N5•Cl
分子量: 311.8
溶解度: DMF: 0.1 mg/ml,DMSO: 0.25 mg/ml,Ethanol: 3 mg/ml,PBS (pH 7.2): 1 mg/ml
储存条件: Store at -20°C
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述：
5-Cyano-2,3-di-(p-tolyl)tetrazolium (chloride) is a redox-sensitive tetrazolium salt used primarily to detect metabolic activity in microorganisms. This membrane-permeable indicator is readily taken up by living cells and reduced by respiratory activity to produce a red fluorescent, water-insoluble formazan crystal (absorption maximum at 450 nm). While the oxidized salt is non-fluorescent, the reduced formazan can be evaluated using a standard rhodamine filter set (excitation: 510-560 nm; emission > 590 nm), although optimum excitation requires a blue 420 nm excitation filter. For flow cytometry, a 480 nm excitation laser combined with detection in the red region is suitable.
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