产品属性:
产品名称 | 4-Nitrophenyl-N-acetyl-β-D-glucosaminide |
规格 | 100mg、250mg、500mg、1g |
货号 | EY-01Y15080 |
Cas No.: 3459-18-5
别名: N/A
化学名: N/A
分子式: C14H18N2O8
分子量: 342.3
溶解度: DMF: 15mg/mL,DMF:PBS (pH 7.2) (1:4): 0.2mg/mL,DMSO: 3mg/mL
储存条件: Store at -20°C
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping ConditionEvaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述:
4-Nitrophenyl-N-acetyl-β-D-glucosaminide is a chromogenic substrate for N-acetyl-β-glucosaminidase.1,2 Hydrolysis of 4-nitrophenyl-N-acetyl-β-D-glucosaminide by N-acetyl-β-glucosaminidase releases 4-nitrophenol, which can be quantified by colorimetric detection at 405 nm as a measure of N-acetyl-β-glucosaminidase activity. It has been used to quantify the activity of N-acetyl-β-D-glucosaminidase in human serum and urine in parenterally fed patients.3|1. Borooah, J., Leaback, D.H., and Walker, P.G. Studies on glucosaminidase. 2. Substrates for N-acetyl-β-glucosaminidase. Biochem. J. 78(1), 106-110 (1961).|2. Bowers, G.N., Jr., McComb, R.B., Christensen, R.G., et al. High-purity 4-nitrophenol: Purification, characterization, and specifications for use as a spectrophotometric reference material. Clin. Chem. 26(6), 724-729 (1980).|3. Raczkowska, K., Zalewska-Szajda, B., Raczkowski, K., et al. The activity of N-acetyl-β-D-hexosaminidase in serum and urine of parenterally fed patients. Exp. Clin. Hepatology 9, 1-4 (2013).
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