产品属性:
产品名称 | Janelia Fluor 549, SE |
规格 | 2 mg |
货号 | EY-01Y20048 |
Cas No.: 1811539-32-8
别名: N/A
化学名: N/A
分子式: C31H25N3O7
分子量: 551.56
溶解度: Soluble in DMSO
储存条件: Store at -20°C
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition:Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述:
Yellow fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells) and STED. Also suitable for flow cytometry. Cell permeable. Excitation maximum = 549 nm; emission maximum = 571 nm;Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1;A280 correction factor is 0.169.We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals.HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat.Methods 12 244 PMID:25599551 |Deng et al (2015) CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells. Proc.Natl.Acad.Sci.USA. 112 11870 PMID:26324940 |Knight et al (2015) Dynamics of CRISPR-Cas9 genome interrogation in living cells. Science 350 823 PMID:26564855 |Legant et al (2016) High-density three-dimensional localization microscopy across large volumes. Nat.Methods 13 359 PMID:26950745
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