产品属性:
产品名称 | Fluorescein |
规格 | 10mM*1 mL in DMSO、250mg、1g、5g |
货号 | EY-01Y20281 |
Cas No.: 7/5/2321
别名: N/A
化学名: N/A
分子式: C20H12O5
分子量: 332.31
溶解度: DMSO: ≥ 100 mg/mL (300.92 mM); H2O: 1 mg/mL (3.01 mM; ultrasonic and warming and heat to 80°C)
储存条件: 4°C, protect from light
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition:Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述:
Fluorescein (Resorcinol phthalein) is a synthetic organic photoactive dye compound. Fluorescein is used as a fluorescent tracer in medicinal and biological applications and tumor infected tissues tracer[1][2].Fluorescein is nearly inactive as a photodynamic sensitizer. Fluorescein consequently is almost useless as a photodynamic sensitizer, but it is of great value in fluorescence immunoassays[1].The fluorescein dye is a common fluorescent probe. Its very high molar absorptivity at the wavelength of the argon laser (488 nm), large fluorescence quantum yield and high photostability makes it a very useful and sensitive fluorescent label. Fluorescein is commercially available in many derivatives, such as fluorescein isothiocyanate and fluorescein succinimidyl ester, that can be covalently attached to macromolecules and to amino acids. The labeled molecules can be detected with very high sensitivity which is utilized in, for example, capillary electrophoresis. The emission spectrum of fluorescein overlaps extensively the absorption spectrum of tetramethyl rhodamine, which is a related strongly fluorescent dye, making this pair very suitable for energy transfer experiments to determine distances within and between labeled macromolecules. Fluorescein in aqueous solution occurs in cationic, neutral, anionic and dianionic forms making its absorption and fluoresence properties strongly pH dependent[3].[1]. Nabel A Negm, et al. Fluorescein dye derivatives and their nanohybrids: Synthesis, characterization and antimicrobial activity. J Photochem Photobiol B. 2016 Sep;162:421-433.
[2]. Li Liu, et al. Fluorescein as an artificial enzyme to mimic peroxidase. Chem Commun (Camb). 2016 Nov24;52(96):13912-13915.
[3]. RobertSjÖback, et al. Absorption and fluorescence properties of fluorescein. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
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