产品属性:
产品名称 | 4-Methylumbelliferyl-β-D-Glucuronide (hydrate) |
规格 | 250mg、500mg、1g、5g |
货号 | EY-01Y20260 |
Cas No.: N/A
别名: N/A
化学名: N/A
分子式: C16H16O9•2H2O
分子量: 388.3
溶解度: DMF: 20 mg/ml,DMSO: 5 mg/ml,Ethanol: 2 mg/ml,PBS (pH 7.2): 2 mg/ml
储存条件: Store at -20°C
General tips:For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition:Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
产品描述:
4-Methylumbelliferyl-β-D-glucuronide is a fluorogenic substrate of β-glucuronidase. 4-Methylumbelliferyl-β-D-glucuronide is cleaved by β-glucuronidase to release the fluorescent moiety 4-methylumbelliferyl (4-MU). 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH, respectively, and an emission maximum ranging from 445 to 455 nm, increasing as pH decreases. 4-Methylumbelliferyl-β-D-glucuronide has been used in the β-glucuronidase reporter system, also known as the GUS reporter system, for fluorescent detection of β-glucuronidase gene expression in E. coli and transformed plants.
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